Journal: ACS Omega

Article Title: Silencing of LncRNA XIST Suppressed Tumor Growth and Metastasis in Papillary Thyroid Carcinoma by Modulating miR-204/FN1 Axis

doi: 10.1021/acsomega.5c00390

Figure Lengend Snippet: Protein expression of EMT-related proteins (E-cadherin, N-cadherin, and Vimentin) in PTC cells with different transfections using Western blot. * p < 0.05, ** p < 0.01 compared with the blank group; # p < 0.05, ## p < 0.01 compared with the siNC + inhibitor NC group; and p < 0.05, and p < 0.01 compared with the si-lncRNA + inhibitor NC group; @ p < 0.05, @@ p < 0.01 compared with the si-lncRNA + miRNA inhibitor group; $ p < 0.05, $$ p < 0.01 compared with the siNC + miRNA inhibitor group; % p < 0.05, %%: p < 0.01 compared with the si-FN1 + inhibitor NC group.

Article Snippet: After blocking with 5% skim milk at 37 °C for 2 h, the membranes were incubated with anti-E-cadherin antibody (1:1000, Proteintech, Wuhan, China), anti-N-cadherin antibody (1:500, Boster, Wuhan, China), anti-Vimentin antibody (1:1000, Proteintech), and anti-GAPDH antibody (1:5000, Proteintech) overnight at 4 °C.

Techniques: Expressing, Transfection, Western Blot

Journal: PLoS ONE

Article Title: Mild Endoplasmic Reticulum Stress Promotes Retinal Neovascularization via Induction of BiP/GRP78

doi: 10.1371/journal.pone.0060517

Figure Lengend Snippet: HRMEC were treated with 10 ng/ml of tunicamycin for 2 h. (A) BiP on the cell surface was precipitated after biotinylation to detect cell surface protein in untreated (control) or tunicamycin treated cells. (B) BiP and T-cadherin were immunoprecipitated with the anti-KDEL antibody to confirm the association of BiP with T-cadherin. Whole lysate without immunoprecipitation with the anti-BiP antibody was similarly evaluated. (C) After the membrane extraction, BiP and T-cadherin were immunoprecipitated with the anti-T-cadherin antibody and detected by using Western blotting with the anti-BiP antibody. Whole lysate without immunoprecipitation with the anti-VEGF receptor-2 (VEGFR-2) antibody was similarly evaluated to confirm the success of the membrane extraction. (D) Immunostaining showed the presence of BiP and T-cadherin on the surface of HRMEC. Scale bar indicates 30 µm.

Article Snippet: Immunoprecipitation products were analyzed by Western blotting using anti-BiP antibody (BD Biosciences, San Diego, CA, USA) and anti-T cadherin antibody (AnaSpec, Fremont, CA, USA).

Techniques: Immunoprecipitation, Western Blot, Immunostaining

Journal: PLoS ONE

Article Title: Mild Endoplasmic Reticulum Stress Promotes Retinal Neovascularization via Induction of BiP/GRP78

doi: 10.1371/journal.pone.0060517

Figure Lengend Snippet: (A) OIR model mice at P17, treated with 3 µg/ml of tunicamycin at P14, were perfused with FITC-dextran and the retinas were stained with anti-BiP antibody. The original images (green channel) and BiP stained images (red channel) are shown, along with the analyzed images. (B) BiP (green channel) and CD31 (red channel) staining in flat-mounted retinas of P17 mice, under normoxia or subjected to the OIR protocol, following tunicamycin injection. Arrowheads and arrows indicate normal vessels and newly formed vessels anterior to internal limiting membrane, respectively. Scale bars indicate 50 µm. The complexes of BiP and T-cadherin in the retina were immunoprecipitated with the anti-T-cadherin antibody and detected by using Western blotting with the anti-BiP antibody (C), or by the Coomassie-staining (D).

Article Snippet: Immunoprecipitation products were analyzed by Western blotting using anti-BiP antibody (BD Biosciences, San Diego, CA, USA) and anti-T cadherin antibody (AnaSpec, Fremont, CA, USA).

Techniques: Staining, Injection, Immunoprecipitation, Western Blot

Journal: New Microbes and New Infections

Article Title: Epidemic of venereal treponematosis in wild monkeys: a paradigm for syphilis origin

doi: 10.1016/j.nmni.2020.100670

Figure Lengend Snippet: Parallel in-clinical course between human syphilis and treponematosis of green monkeys. Chancre was on penis of a green monkey (Niokolo forestry guardhouse) sampled in November 2015 (A09) and was PCR positive for Treponema pallidum. Visualization of T. pallidum (brown spirochetes) using immunohistochemical detection (original magnification ×400) was based on biopsy results of penis lesion found on baboon sampled in August 2016 (BNK14). Positive Western blot serology was obtained from green monkey sampled in August 2016 (SNK21).

Article Snippet: Anti– T. pallidum Western blot analyses (Euroimmun, Lübeck, Germany) were performed according to the manufacturer's instructions.

Techniques: Immunohistochemical staining, Western Blot

Journal: New Microbes and New Infections

Article Title: Epidemic of venereal treponematosis in wild monkeys: a paradigm for syphilis origin

doi: 10.1016/j.nmni.2020.100670

Figure Lengend Snippet: Pairwise comparison of number of single nucleotide polymorphisms between different strains of Treponema pallidum

Article Snippet: Anti– T. pallidum Western blot analyses (Euroimmun, Lübeck, Germany) were performed according to the manufacturer's instructions.

Techniques: Comparison

Journal: New Microbes and New Infections

Article Title: Epidemic of venereal treponematosis in wild monkeys: a paradigm for syphilis origin

doi: 10.1016/j.nmni.2020.100670

Figure Lengend Snippet: Median joining network (MJN) representing relationships between different strains of subspecies of Treponema pallidum based on single nucleotide polymorphisms. Each circled area indicates unique genotype. Number of mutation steps between strains is indicated in red.

Article Snippet: Anti– T. pallidum Western blot analyses (Euroimmun, Lübeck, Germany) were performed according to the manufacturer's instructions.

Techniques: Mutagenesis